柏业贸易

概述

CustomAccuPower®PCR PreMix Service产品的优势在于，通过最大限度地减少非特异性扩增产物和引物二聚体的形成，提高了反应特异性和PCR扩增效率。可以订购用于各种类型的PCR反应的预混物，例如复杂的基因组学，cDNA模板，低拷贝靶标，多重PCR等。此外，将热启动PCR所需的成分（例如DNA聚合酶，dNTP，反应缓冲液，稳定剂和热稳定的PPase）与客户定制的引物组混合，并按一次使用量分装干燥，仅添加模板和D.W.便可进行PCR反应。

特点及优势

稳定性
使用专利稳定剂，酶活性可在室温下保持一个月，冷冻时可保持1年
特异性
每个PCR管均包含客户所需的引物组，DNA聚合酶以及PCR反应所需的所有成分，只需添加DNA和D.W.即可立即进行PCR反应。由于包含了电泳所需的示踪染料和沉淀剂，因此无需添加sample loading buffer，并最大程度减少气溶胶产生带来的污染
重现性
ISO 9001质量体系下的批量生产可最大程度地减少批次之间的差异，即使在重复实验中也可得到可重复的实验结果

实验数据

Figure. Custom AccuPower® PCR PreMix: Reproducibility & Sensitivity. Custom AccuPower® DsRed2 HotStart PCR PreMix was tested three times, A-1, A-2 and A-3, with the condition of: 1. 94°C 2 min; 2. 30 cycles of 94°C 30 sec, 58°C 30 sec and 72°C 30 sec; 3. 72°C 3 min. 5 ul of amplified PCR product was run on a 2% TBE agarose gel.

Lane 1; 20-2x positive DNA. Lane 2; 20-3x positive DNA, Lane 3; 20-4x positive DNA,
Lane 4; 20-4x positive DNA, Lane N; Negative control, Lane M; 100bp DNA Ladder (D-1030)

客户

Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA, Korea)
Korea Centers for Disease Control and Prevention (Korea)
Korea National Institute of Health (Korea)
Chungnam National Institute of Public Health and Environment (Korea)
The National plant quarantine service (Korea)
National Agricultural Products Quality Management Service (Korea)
National Fishery Products Quality Management Service (Korea)
Research Center for Risk of LMO in Pukyong National University (Korea)
Research Center for Resistant Cells in Chosun University (Korea)
PKPORK (Korea)
Seoul Song Do Colorectal Hostpital (Korea)
Purigo company (Taiwan)
Hylab company (Israel)
Kirkhouse company (U.K) etc..

▶ Specifications

▶ Custom AccuPower® Products with PCR PreMix Service

ILivestock Related Products

• Custom AccuPower® PCV2 HotStart PCR PreMix
• Custom AccuPower® PRRS HotStart RT-PCR PreMix
• Custom AccuPower® PRRS Nested HotStart PCR PreMix
• Custom AccuPower® Q-Fever HotStart PCR PreMix
• Custom AccuPower® Brucella HotStart PCR PreMix
• Custom AccuPower® Cva A/B, fyuA, FIMC 3-Mutliplex HotStart PCR PreMix
• Custom AccuPower® Vat, iucD, Irp2 3-Mutliplex HotStart PCR PreMix
• Custom AccuPower® Tsb, Iss, astA 3-Mutliplex HotStart PCR PreMix
• Custom AccuPower® ConOri, CsPcSC43528 & STM3664 3-plex HotStart PCR PreMix
• Custom AccuPower® CPA, CPB, CPE & CPI 4-plex HotStart PCR PreMix
• Custom AccuPower® E. Coli EAST1 HotStart PCR PreMix
• Custom AccuPower® E. Coli PAA HotStart PCR PreMix
• Custom AccuPower® E. Coli AIDA-1 HotStart PCR PreMix
• Custom AccuPower® Lowsonia HotStart PCR PreMix
• Custom AccuPower® Brachyspira HotStart PCR PreMix
• Custom AccuPower® HPS, APP & A.Suis 3-Multiplex HotStart PCR PreMix
• Custom AccuPower® tcdB HotStart PCR PreMix
• Custom AccuPower® RVD1 HotStart RT-PCR PreMix
• Custom AccuPower® RVD2 HotStart RT-PCR PreMix
• Custom AccuPower® JEE HotStart RT-PCR PreMix
• Custom AccuPower® Salmonella HotStart PCR PreMix
• Custom AccuPower® EAE HotStart PCR PreMix
• Custom AccuPower® Malaria rVIV HotStart PCR PreMix
• Custom AccuPower® Clostridium pefrigens HotStart PCR PreMix
• Custom AccuPower® Malaria rPLU5/6 HotStart PCR PreMix
• Custom AccuPower® cdtA HotStart PCR PreMix
• Custom AccuPower® cdtB HotStart PCR PreMix
• Custom AccuPower® tcdA HotStart PCR PreMix
• Custom AccuPower® Neospora HotStart PCR PreMix
• Custom AccuPower® Toxoplasma HotStart PCR PreMix
• Custom AccuPower® Leptospra HotStart PCR PreMix etc.

IIGMO & Plant Related Products

• Custom AccuPower® P. destructiva HotStart PCR PreMix
• Custom AccuPower® P. exigua HotStart PCR PreMix
• Custom AccuPower® P. glomerata HotStart PCR PreMix
• Custom AccuPower® P. loticola HotStart PCR PreMix
• Custom AccuPower® P. medicaginis HotStart PCR PreMix
• Custom AccuPower® CMM5/6 HotStart PCR PreMix
• Custom AccuPower® CaMv p35s Multiplex PCR PreMix
• Custom AccuPower® NOS Multiplex PCR PreMix
• Custom AccuPower® DsRed Multiplex PCR PreMix
• Custom AccuPower® PB3/4 HotStart PCR PreMix etc.

IIIFish Related Products

• Custom AccuPower® White spot syndrome virus(146F1/R1) HotStart PCR PreMix
• Custom AccuPower® White sopt syndrome virus(146F1/R2) HotStart PCR PreMix
• Custom AccuPower® SVCV F1/R1 HotStart PCR PreMix
• Custom AccuPower® SVCV F1/R4 HotStart PCR PreMix etc.

IVOthers

• Custom AccuPower® D8M309 HotStart PCR PreMix
• Custom AccuPower® D11M5 HotStart PCR PreMix
• Custom AccuPower® D13M78 HotStart PCR PreMix
• Custom AccuPower® D14M14 HotStart PCR PreMix
• Custom AccuPower® D15M226 HotStart PCR PreMix
• Custom AccuPower® D18M64 HotStart PCR PreMix
• Custom AccuPower® D19M19 HotStart PCR PreMix
• Custom AccuPower® DXM210 HotStart PCR PreMix
• Custom AccuPower® BID HotStart PCR PreMix
• Custom AccuPower® rPLU 5/6 (1st)HotStart PCR PreMix
• Custom AccuPower® rVIF F/R (2nd) HotStart PCR PreMix

▶ Experimental data

• Custom AccuPower® Hotstart PCR PreMix

Custom AccuPower® Hotstart PCR PreMix is manufactured / produced by the customer's manufacturing method and Bioneer Premix patent, and the optimal sensitivity test is completed.

• Custom AccuPower® Multiplex PCR PreMix

Custom AccuPower® Multiplex PCR PreMix is manufactured / produced by the customer's manufacturing method and Bioneer Premix patent, and the optimal sensitivity test is completed.