柏业贸易

特点及优势

便利性
简化的操作流程，可在一个管中连续进行cDNA合成和PCR反应，仅需添加模板RNA及其特异性引物
稳定性
添加稳定剂和RNase抑制剂以提高抗降解能力
重现性
ISO 9001质量体系下的批量生产可最大程度地减少批次之间的差异，即使在重复实验中也可得到可重复的实验结果

应用

• First-strand synthesis of cDNA from RNA molecules (RT)

• RT-PCR

• cDNA library construction

• Gene-expression analysis

实验数据

rt pcr figure1

Figure. Specific amplification of 5'- UTR region of HCV with AccuPower® RT/PCR PreMix.
Specific amplification of 5' - UTR region of HCV with AccuPower® RT-PCR PreMix. Human DNA was amplified to generate 0.1-1.5 kb PCR fragments using 1 U of polymerase. The comparison was performed under the same experimental conditions.

Lane 1; DNA Ladder (BM corp.)
Lane 2; negative control
Lane 3, 4; HCV positive serum
Lane 5; HCV negative serum

▶ Specifications

▶ Experimental data

rt pcr figure1

Figure 1. Sensitivity comparison between AccuPower® RT/PCR PreMix and Supplier I RT-PCR Kit.
Each 109 copy – 103 copy of PSTVd used for RT/PCR and the same amount of RT/PCR products used for electrophoresis

Lane1; 109 copy Lane2; 108 copy Lane3; 107 copy Lane4; 106 copy
Lane5; 105 copy Lane6; 104 copy Lane7; 103 copy Lane8; NTC

rt pcr figure1

Figure 2. Comparison of reproducibility test for AccuPower® RT/PCR PreMix batch 1,2,3 and batch 4 products using serial diluted human total RNA.

Lane 1; 10 ng human total RNA from HeLa cell
Lane 2; 1 ng human total RNA from HeLa cell
Lane 3; 100 pg human total RNA from HeLa cell
Lane 4; 10 pg human total RNA from HeLa cell