特点及优势
便利性
包含了cDNA合成所需的所有组分,仅添加模板RNA和引物即可轻合成cDNA
稳定性
添加稳定剂和RNase抑制剂以提高抗降解能力
重现性
ISO 9001质量体系下的批量生产可最大程度地减少批次之间的差异,即使在重复实验中也可得到可重复的实验结果。
应用
• First-strand synthesis of cDNA from RNA molecules (RT)
• Random priming reaction
• cDNA library construction
• Probe labeling
• mRNA 5’-end mapping by primer extension analysis
• PCR
• Real-time qPCR
实验数据
Figure. Amplification of an GAPDH target gene was detected using human total RNA (from 10 ng to 10 pg ) with AccuPower® RT PreMix.
Lanes 1-4; 10 ng, 1 ng ,and 100 pg, 10 pg of total RNA from HeLa cells, respectively.