柏业贸易

特点及优势

高保真
通过高保真度在DNA扩增过程中实现低突变率（错误率= 1.9X10-6）(error rate= 1.9X10-6)
灵敏度
出色的灵敏度和扩增效率亦可检测痕量样品
应用广
有效扩增大靶标，可进行多种应用，如启动子检测和克隆实验
稳定性
添加了稳定剂可以在常温下运输，比溶液型产品更稳定
便利性
只需加入模板DNA，引物即可，轻松上手
重现性
ISO 9001质量体系下的批量生产可最大程度地减少批次之间的差异，即使在重复实验中也可得到可重复的实验结果

应用

• Gene synthesis

• Gene cloning

• Conventional PCR

• Primer extension

• Site-directed mutagenesis

• High fidelity PCR

实验数据

Figure. Template Range & Sensitivity test
Test of working range & sensitivity of AccuPower® Pfu PCR PreMix for Lambda DNA template.

M; 100 bp DNA Ladder (Cat. No. D-1030, Bioneer)
Line 1; 10ng
Line 2; 1ng
Line 3; 100pg
Line 4; 10pg
Line 5; 1pg
Line 6; 100 fg
Line 7; 10 fg
Line 8; template negative

▶ Specifications

Enzyme	Pfu DNA polymerase
5' to 3' exonuclease	No
3' to 5' exonuclease	Yes
3'- A overhang	No
Fragment Size	Up to 15 kb

▶ Experimental data

Figure 1. Test of working range & sensitivity of AccuPower^® Pfu PCR PreMix for human DNA template.
Test of working range & sensitivity of AccuPower^® Pfu PCR PreMix for Lambda DNA template.

M; 100 bp DNA Ladder (Cat. No. D-1030, Bioneer)
Line 1; 100 ng Line 4; 100 pg
Line 2; 10 ng Line 5; 10 pg
Line 3; 1 ng Line 6; template negative

Figure 2. Amplification of Lambda DNA of 1 kb to 10 kb with AccuPower^® Pfu PCR PreMix.

M; 1 kb DNA Ladder (Cat. No. D-1040, Bioneer)
Line 1; 1 kb fragment Line 6; 6 kb fragment
Line 2; 2 kb fragment Line 7; 7 kb fragment
Line 3; 3 kb fragment Line 8; 8 kb fragment
Line 4; 4 kb fragment Line 9; 9 kb fragment
Line 5; 5 kb fragment Line 10; 10 kb fragment