Overview
Optical Patent Technology
Bioneer patented fluorescence detection method (Korean Patent No. 10-1089045, US Patent No. US 8427643) was applied to minimize noise caused by reflected light, thus improving sensitivity and accuracy.
Figure. Separation of a reflection light from fluorescence
* Noise caused by reflected light: The fluorescent materials used in qPCR create emission when the absorbed excitation energy excites them then falls to the ground state. In conventional qPCR instruments, part of excited light irradiated from the sample is reflected from the surface of the plate and the instrument, which may enter the detector to be also mistakenly read as part of the light from the sample. As the excited light has much more luminance than the fluorescent light, reflected light also contain high luminance, making it unable to be completely removed by the fluorescent light and creating noise in the process, deteriorating sensitivity and accuracy. However, by using our patented technology, excitation and fluorescence have different polarity, allowing the reflect light unable to be blocked by fluorescent filter to be filtered once again through polarizing filter. This allows drastic reduction of noise caused by reflected light compared with other qPCR instruments.
Features and Benefits
High reproducibility and accuracy
Highly reproducible and accurate results by light tunnel (LT) technology (Korean Patent No. KR 0794703, US Patent No. 8139210, Japanese Patent JP 4751821, China Patent No. CN 1798969) irradiating uniform excitation light, excitation light interception method (Korean Patent No. 10-1089045 and US Pat. No. 8427643) using polarization, and a 96-well simultaneous detection technique using a 2-D sensor minimizing optical sensitivity and deviation between well
Reduce reaction time
Maximum ramping rate of 4.5 ℃/sec (Exicycler ™ 96 Fast: max 5.0 ℃/sec) decreases reaction time by using a unique thermal block and temperature control technology
5 color multiplexing system
Simultaneous detection of five targets capable of being used in multiplex PCR without the need of reference dyes, used for the compensation of light quantity deviation, through the uniform optical system patent technology (Korean Patent KR 0794703, US Patent US 8139210, Japanese Patent JP 4751821, China Patent CN 1798969)
Convenience
Diverse choice of fluorescent dyes including the universal ones with wide excitation/emission wavelength detection range being 475 to 690nm, unlike other traditional instruments designed for specific dyes
Wide dynamic range
Large detection range being more than 9 log allowing DNA detection and qualitative analysis even at low and high concentration samples using a short Arc lamp, a powerful excitation light source
Temperature gradient function
Simplified search of optimal PCR annealing temperature with temperature gradients from 1~20°C available in the range of 20~95°C
Various application
Wide applications such as touch down PCR through time increment and temperature increment adjustment functions
User-friendly software
Accessible User-centric GUI for easy operation and manipulation for designing experiments and analyzing results
Application
• Gene expression analysis
• MicroRNA expression analysis
• Gene copy number analysis
• Pathogen detection
• Genetic mutation test
• GMO quantitative and qualitative test
